How to Improve Your Viral Nucleic Acid Extraction Processes
Thu 16 Apr, 2020
The extraction of DNA or RNA is a crucial step for the successful detection of viruses. Due to numerous epidemics in recent decades, the demands on diagnostics have increased worldwide. Laboratories are confronted with more and more samples that have to be processed in the same time. To speed up your virus diagnostics, Analytik Jena has developed a new solution for high-throughput extraction - in a user-friendly way for the benchtop. In this article you can find out exactly how the CyBio FeliX Extraction Set simplifies your virus extraction.
High-Throughput Extraction with CyBio FeliX
The CyBio FeliX liquid handling platform was optimized by Analytik Jena for high-throughput extraction of nucleic acids. With this system you can achieve considerably faster results and higher result quality when extracting viral RNA. Analytik Jena offers a special CyBio FeliX Extraction Set, a complete solution consisting of hardware, kits and software tailored for extraction. With this set you can purify up to 96 samples in parallel and fully automated. Predefined extraction applications make your work easier. Simply select the protocol for virus extraction in the software and start your application with a few clicks - time-consuming programming is not required. The CyBio FeliX is available in a Clean Bench version on request - the Extraction Set also works with this model. The CyBio FeliX and the CyBio FeliX Extraction Set are designed for high throughput extraction applications. If you want to process a lower number of samples, Analytik Jena offers you more specialized solutions. For example, the InnuPure C16 touch and optimized extraction kits are available the flexible extraction from 1-16 samples. The kits use the same extraction chemistry as the kits for the CyBio FeliX.
High-Throughput Extraction of Viral RNA from Respiratory Swabs
The innuPREP Virus TS RNA Kit 2.0 FX is an extraction kit for viral RNA from respiratory swabs. The kit is available –as package size for 96 reactions. The elution volume is between 50 and 200 µl. The kit is designed for use with the CyBio FeliX and provides you with the fastest results and best yield. The extraction time is 62 minutes. The yield depends on the quality of the starting material and the viral load, but usually exceeds the yield of conventional methods considerably. Avoid freezing and thawing your samples for the highest possible yield. The kits are designed for sample volumes of 200 µl (isolation from swabs stored under physiologic saline) and 400 µl (direct isolation from swab sample). For optimal results, you should therefore ensure that these volumes are maintained.
Everything - Prefilling & Consumables
The innuPREP Virus TS RNA Kit 2.0 FX is not available prefilled. You must transfer the reagents to the appropriate wells of the plates prior to the extraction process. You can fill the reagents either manually or automatically. With the innuPREP Prefilling Set, Analytik Jena offers you a convenient solution for automated prefilling with the CyBio FeliX. The prefilling protocols required are already predefined and available in the software. Necessary hardware components are also already included in the CyBio FeliX Extraction Set. Manual prefilling takes about 15 minutes for 96 samples. Depending on the available lab equipment and number of samples, this number may vary slightly. The automated prefilling takes about 50 minutes. Appropriate plasticware is included in the kit as well. All plastics are adapted to the extraction application. You may use tube racks in 96-well format from other manufacturers. However, they should be in the standardized SLAS format.
Click and Extract - Application Studio CyBio FeliX extract
The Application Studio CyBio FeliX eXtract simplifies your extraction with intuitive controls and many comfort features. The software is designed to make your work experience as easy as possible. You simply select the kit to be used, enter the start and elution volume, press “Start” and your extraction workflow begins. The respective walk-away time is displayed in the user interface. The loading of the CyBio FeliX with the plates (deck layouts) is explained in the software with simple images and text. Additionally, you can read the explanations in the kit manual.
Overview of Hardware and Kits
CyBio FeliX Extraction Set
- 1x CyBio head R 96/1000 µl, incl. cover magazine
- 3x 96 channel magazine for head R 96/1000 µl
- 3x support; 97 mm height
- 1x 8 channel adapter for head R 96/1000 µl, incl. support (37mm height)
- 1x CyBio FeliX Gripper, incl. support (37mm height)
- 1x magnet adapter Magnum FLX Enhanced
- 1x BioShake 3000-T elm, incl. adapter for deep well plates
- 1x waste box
- 1x tip transfer tool 96/1000 µl
- 1x tip rack 96/1000 µl
- Control accessories (cable, USB hub, adapter)
- Application Studio CyBio FeliX extract (software) including predefined protocols
innuPREP Virus TS RNA Kit 2.0 FX
- Respiratory swab samples as starting material
- Extraction time 62 minutes
- Elution volume 50 -200 µl
- Very high yield (depending on the quality of the starting material)
Prefilling & Consumables
- innuPREP Prefilling Set for 5x 96-well reprocessing
- Hardware included in CyBio FeliX Extractions set
- Automated prefilling time approx. 50 min
- Manual prefilling depending on the number of samples and equipment
- Set contains optimized plastic ware
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