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Configuration examples for MALDI-TOF-MS sample preparation

Standard MALDI sample preparation

Capacity

  • 1x MALDI plate
  • 1-4x sample plate(s)

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x Tip wash station wash buffer
  • 1x MALDI plate
  • 1-4x source plate(s)

Extended standard MALDI sample preparation

Including plate storage

Capacity

  • CyBio QuadStack M: 90 MALDI plates or 30 source plates (1536) and 30 MALDI plates or 40 source plates (384) and 10 MALDI plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-30x MALDI plates
  • 1-40x source plate(s)

Fully automated MALDI-TOF sample preparation

Including storage for source and MALDI plates

Capacity

  • Plate storage - Hotel: 10 x 21 = 210 sample plates
  • CyBio QuadStack M: 90 MALDI plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-90 x MALDI plates

1-210 x source plate(s)

CB-Vario deck layout

  1. MALDI plate
  2. Source plate
  3. Matrix reservoir
  4. De-ionisation
  5. Tip touch station
  6. Tip wash

Extended fully automated MALDI-TOF sample preparation

Including plate store, sealer, peeler, centrifuge

Capacity per batch

  • 90 MALDI target plates (CyBio QuadStack (M) 3 racks) and 180 (CyBio QuadStack (L) 3 racks) source plates

Throughput

  • < 10 min to prepare a MALDI target plate

Start situation

  • 1x Matrix reservoir
  • 1x TWS wash buffer
  • 1-90 x MALDI plates
  • 1-180 x source plate(s)

MALDI-TOF reader plate delivery set up

Including CyBio QuadStack M

Capacity

  • 1 - 90 plates

Throughput

  • 10 s for plate handling

Start situation

  • MALDI plates sorted in the CyBio QuadStack M

Including random accesses plate storage

Capacity

  • 1 - 110 plates

Throughput

  • 10 s for plate handling

Start situation

MALDI plates sorted in the shelf

Components

Analytik Jena Devices
ComponentDescription
CyBio® Well vario PipettorBase station without pipetting head; combine with a circular transport system and a pipetting head to form a functional system
10 position circular transport system

Circular transport system with base frame and rotary table with 10 positions

CyBio® Well vario Pipetting head with 1536/8 μl or 384/25 μl Tip MagazineCyBio® Well vario Pipetting Head for 384- or 1536-well simultaneous liquid handling and Fixed Tip Magazine 1536/1 µl or 384/25 µl Ceramic with individually changeable non-disposable ceramic tips
CyBio® Well vario Barcode reader NLV 3101For reading barcode labels on the short side of microplates on linear transport system
Pump modulePump module with 2 pumps and power supply
Tip wash station 1536Complete tip wash station 1536 set
MALDI sample adapter 1536For optimal dry transfer of small volumes. Optimized for Bruker MALDI sample plate/MALDI microplates with 127.7 mm x 85.4 mm footprint
Microplate adapter 1536For use with 1536-well microplates with CyBio® Well vario
Reservoir 1536Optimized for applications with CyBio® Well vario Pipetting Head, 1536 with refillable option on request
Active dryerPark deck and vacuum chamber to reduce the drying time for MALDI sample plate
CyBio® QuadStack MMedium sized stacker with capacity for 30 SW plates per stack, MALDI sample plate storage
Third Party example devices
ComponentDescription
MS readerHigh speed mass spectrometer
Robotic arm4 Axis Scara robotic arm with 750 mm vertical stroke, servo gripper
IncubatorStorage of assay plates (optional with lid) with a capacity for 210 shallow well plates
PeelerFully automated seal removal in wide variety of environments
SealerThermal microplate sealer, accommodates microplates as tall as 63.5 mm, sealing temperature 30 to 200°C, cycle time ~ 8 seconds per plate
CentrifugeMicro plate centrifuge for up to 5000 x g, imbalance tolerant: up to 100 g weight difference between buckets, 2-bucket rotor, maximum plate height: 61 mm
HotelCarousel hotel package, 10 place, 220 capacity, package with 10 extended-height random access hotels

 

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